Micromorphological and Nutritional Attributes of two Varieties of Vernonia amagdalina Del. Domesticated in Delta State
Keywords:
Micromorphological, nutritional characteristics, Vernonia amygdalina, Agbor, Ika South, Delta StateAbstract
Authors: Thelma Ewere Konyeme and Anthony Ossai Ukpene
Received : 12 May 2024/Accepted: 12 September 2024
This study examined the potential of two Vernonia amygdalina varieties (bitter and non-bitter) as sources of nutrients and medicinal compounds. Micromorphological analysis revealed variations in stomata size (bitter: 1.57±0.20 μm; non-bitter: 1.49±0.42 μm), index (bitter: 1.20±0.60%; non-bitter: 2.30±0.21%), and trichome index (bitter: 0.55±0.36%; non-bitter: 0.90±0.19%) between the varieties, while stomata type and anticlinal wall patterns were similar. Both varieties were rich in minerals, with bitter varieties containing higher levels of sodium (7.27±0.43 mg/g), potassium (17.07±0.45 mg/g), phosphorus (3.15±0.41 mg/g), iron (0.65±0.02 mg/g), zinc (0.15±0.03 mg/g), and copper (0.16±0.04 mg/g) compared to non-bitter varieties. Proximate composition analysis showed higher levels of crude fiber (1.76±0.50%), crude protein (21.07±0.30%), and carbohydrate (23.33±0.82%) in non-bitter varieties, while bitter varieties had higher levels of moisture (50.01±0.40%), crude ash (7.95±0.56%), and crude fat (2.15±0.20%). Phytochemical analysis revealed higher levels of tannin (0.13±0.85%), saponin (0.08±0.02%), and flavonoid (0.22±0.09%) in non-bitter varieties, while alkaloid levels (1.26±0.27%) were higher in bitter varieties. These findings suggest that both Vernonia amygdalina varieties are valuable sources of nutrients and phytochemicals, with potential applications in food and medicine.
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References
Adedeji, O., Ajuwon, O.Y, & Babawale, O.O. 2007. Foliar epidermal studies, organographic distribution and taxonomic importance of trichomes in the family Solanaceae. International Journal of Botany, 3, 3, , pp. 276-282.
Akinpelu, D.A. 1999. Antimicrobial activity of Vernonia amygdalina leaves. Fitoterapia. 70, pp. 232–234.
Ali, M., Muazu, L., Diso, S. U. and Ibrahim, I. S. 2020. Determination of proximate, phytochemicals and minerals composition of Vernonia amygdalina (bitter leaf). Nutraceutical Res. 1, 1, pp.1–8.
A.O.A.C. 1999. Association of Official Analytical Chemist. Methods of analysis, Washington DC., U.S.A.
Asuzu, U.C. 2020. Anatomical studies of the midrib, petiole and epidermal strip of some Vernonia species from Nigeria. International Journal of Botany, 16, pp. 9-19.
Aworinde, D.O., Ogundairo, B.O. & Erinoso,S.M. 2013. Comparative leaf architectural studies of some Vernonia SCHREB (Asteraceae) in Nigeria. Current Botany, 4, pp 43-47
Bhattacharjee, B., Lakshminarasimhan, P., Bhattacharjee, A., Agrawala, D. K., & Pathak, M.K. 2013. Vernonia amygdalina Delile (Asteraceae)–An African medicinal plant introduced in India. Zoo’s Print, 28, 5, pp. 18–20.
Cheng, H. Y., Lin, C. C. & Lin, T. C. 2002. Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antivir. Res. 55, 3, , pp.447–455.
Cimanga, R.K., Tona, L., Mesia, K., Musuamba, C.T., De Bruyne, T., Apers, S., Hernan, N., Miert, V.S., Pieters, L., Totte, J. & Vlietink, A.J. 2004. In vitro antiplasmodia x ford of extravts and fractions of seven medicinal plants used in the democratic republic of Congo. J. Ethnopharmacol. 93, pp. 27–32.
Danladi, S., Hassan, M. A., Masa’ud, I. A., & Ibrahim, U. I. 2018. Vernonia amygdalina Del: a mini review. Res. J. Pharm. Technol. 11, 9, pp. 4187–4190.
Echem, O. G., & Kabari, L. G. 2013. Heavy metal content in bitter leaf (Vernonia amygdalina) grown along heavy traffic routes in Port Harcourt. Agric. Chem., pp. 201–210.
Etta, H. E., Obinze, A. E. & Osim, S. E. (2017). Proximate composition of five accessions of Vernonia amygdalina (del) in south eastern Nigeria. NISEB J. 14 (4).
Etukudo, M.M., Hamilton-Amachree, A., & Roberts, E. M. I. 2015. Eco-physiological Studies of Elemental and Proximate Contents of Gnetum africanum Welw and Telfairia occidentalis Hook seeds from two Ecological Zones of Akwa Ibom State. European International Journal of Science and Technology, 4, 6, pp. 47-53.
Hladik, C., Krief, S. & Haxaire, C. 2005. Ethnomedicinal and bioactive properties of plants ingested by wild chimpanzees in Uganda. J. Ethnopharmacol. 101, pp.1–5.
Garba, Z. N. & Oviosa, S. 2019. The effect of different drying methods on the elemental and nutritional composition of Vernonia amygdalina (bitter leaf). J. Taibah Univ. Sci. 13, 1, pp. 396–401.
Igile, G.O., Oleszek, W., Burda, S. & Jurzysta, M. 1995. Nutritional assessment of Vernonia amygdalina leaves in growing mice. J. Agr. Food Chem. 43, pp. 2162–2166.
Ijeh, I. & Ejike, C. E. 2011. “Current perspectives on the medical potential of Vernonia amygdalina Del”. Journal of Medical and Plant Research, 5, 7, , pp. 1051-1061.
Degu, S., Meresa, A., Animaw, Z., Jegnie, M., Asfaw, A., & Tegegn, G. (2024). Vernonia amygdalina: A comprehensive review of the nutritional makeup, traditional medicinal use, and pharmacology of isolated phytochemicals and compounds. Frontiers in Natural Products, 3. https://doi.org/10.3389/fntpr.2024.1347855
Ofori, D. A., Anjarwalla, P., Jamnadass, R., Stevenson, P. C., & Smith, P. 2013.Pesticidal plant leaflet Vernonia amygdalina Del. Australia: Royal botanic garden, pp. 1–2.
Okanume, O. E., Abok, S.G. & Oso, O. A. 2022. Leaf epidermal and petiole anatomical features as taxonomic characters in some Vernonia species in Nigeria. Bio-Research, 20, 1, , pp.1388-1397
Okeke, C. U., Ezeabara, C. A., Okoronkwo, O. F., Udechukwu, C. D., Uka, C. J., & Bibian, O. A. 2015. Determination of nutritional and phytochemical compositions of two variants of bitter leaf (Vernonia amygdalina Del). J. Hum. Nutr. Food Sci. 3, 3, , pp. 1065.
Okolie, H., Ndukwe, O., Obidiebube, E., Obasi, C., & Enwerem, J. 2021. Evaluation of nutritional and phytochemical compositions of two bitter leaf (Vernonia amygdalina) accessions in Nigeria. Int. J. Res. Innovation Appl. Sci. 6, 12, , pp. 2454–6194.
Olowoyeye, O. J., Sunday, A., Abideen, A. A., Owolabi, O. A., Oluwadare, O. E. & Ogundele, J. A. 2022. Effects of vegetative zones on the nutritional composition of Vernonia amygdalina leaves in ekiti state. Int. J. Med. Pharm. Drug Res. 6, 2, , pp. 29–34.
Olumide, M. D., Ajayi, O. A., & Akinboye, O. E. 2019. Comparative study of proximate, mineral and phytochemical analysis of the leaves of Ocimum gratissimum, Vernonia amygdalina and Moringa oleifera. J. Med. Plants Res. 13, 15, , pp. 351–356.
Omoyeni, O. A., Olaofe, O. & Akinyeye, R. O. 2015. Amino acid composition of ten commonly eaten indigenous leafy vegetables of South-West Nigeria. World J. Nutr. Health, 3, 1, pp. 16–21.
Metcalf e, C.R. & Chalk, L. (1979). Anatomy of the Dicotyledons: Systematic Anatomy of Leaf and Stem, with a Brief History of the Subject. 2nd Edition. Clarendon Press, Oxford, pp.304-305
Niger Delta Budget. 2024. Overview of Delta Statehttps://www.nigerdeltabudget.org/overview-of- delta/
Niger Delta People. 2023. Ughelli Kingdom. https://nigerdeltapeople.com/ughelli-kingdom/
Nursuhaili, A. B., Nur Afiqah Syahirah, P., Martini, M. Y., Azizah, M. & Mahmud,T. M. M. 2019. A review: medicinal values, agronomic practices and postharvest handlings of Vernonia amygdalina. Food Res. 3, 5, , pp. 380–390.
Ojiako, O.A & Nwajo, H.U. 2005. Is Vernonia amygdalina hepatotoxic or hepatoprotective? Response from biochemical and toxicity studies in rats. Afr. J. Biotechnol.5, pp.1648–1651.
Senthilkumar, A., Karuvantevida, N., Rastrelli, L., Kurup, S. S., & Cheruth, A. J. (2018). Traditional uses, pharmacological efficacy, and phytochemistry of Moringa peregrina (Forssk.) Fiori—A review. Frontiers in Pharmacology, 9, 465. https://doi.org/10.3389/fphar.2018.00465
Tian, B., Liu, J., Liu, Y. & Wan, J. B. 2023. Integrating diverse plant bioactive ingredients with cyclodextrins to fabricate functional films for food application: a critical review. Crit. Rev. Food Sci. Nutr. 63, 25, , 7311–7340.
Ugwu, O. P. C., Nwodo, O. F. C., Joshua, P. E., Abubakar, B., Ossai, E. C. & Odo, C. E. 2013. Phytochemical and acute toxicity studies of Moringa oleifera ethanol leaf extract. Int. J. Life Sc. Bt Pharm. Res. 2, pp. 2250–3137.
Usunobun, U. & Okolie, N. P. 2015. Phytochemical, trace and mineral composition of VA leaves. Int. J. Biol. Pharm. Res. 6, 5, , 393–399.
Usunomena, U. & Ngozi, O. P. 2016. Phytochemical analysis and proximate composition of Vernonia .amygdalina. Int. J. Sci. World, 4, 1, , pp. 11–14.
Yeap, S. K., Ho, W. Y., Beh, B. K., Liang, W. S., Ky, H., Yousr, A. H. N., et al. 2010. Vernonia amygdalina, an ethnoveterinary and ethnomedical-used green vegetable with multiple bioactivities. J. Med. plants Res. 4, 25, pp. , 2787–2812.
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