Isolation, Characterization and Antimycobacterial Potency of a Steroidal Derivative from the Chloroform Crude Extract of Icacina trichantha Oliv Tuber

Abstract

Literature has shown that the extract of Icacina trichantha is rich in various constituents, whose medicinal values have been confirmed. However, the major challenge is in the isolation of the component of interest. In this study, a steroidal derivative was isolated from the tubers of Icacina trichantha. The extraction was done using the cold maceration method, while further isolation and purification were carried out using column chromatography and thin-layer chromatography. Characterization was done using FT-IR, 1HNMR, 13CNMR, and COSY with literature which confirmed the isolated compound to be a steroidal derivative. The antimycobacterial activity was done on the isolated steroidal derivation, and the result obtained confirmed the speculation of its use for the treatment of “tough cough” by the locals. The findings from the study is remarkable in contributing information to phytochemistry.