Volatile Constituents of the Leaves and Stem of Justicia secunda Vahl


  • Edet O. Odokwo Federal University of Otuoke, Bayelsa State, Nigeria


Volatile constituents, Justicia secunda Vahl, GCMS, applications


Communication in Physical Sciences, 2020, 6(2):827-834

Authors: Edet O. Odokwo and Martha S. Onifade

Received 19 December 2020/Accepted 27 December 2020

Volatile constituents of leaves and stem of Justicia secunda Vahl have been investigated. The respective plant parts were extracted using hydrodistillation techniques and the volatile constituents were quantitatively analysed with Gas chromatography coupled to a mass spectrophotometer (GCMS). The results deduced from the spectra of the plant stem and leaf indicated the presence of twenty-four volatile compounds consisting of two (2) monoterpenoids, four (4) sesquiterpenes, one (1) sesquiterpenoid and seventeen (17) non-terpenoids. The isolated compounds and their concentrations were diethyl phthalate (28.71%), bis(2-ethylhexyl) phthalate (52.52%), (3aR, 4R, 7R)- 1,4,9,9-tetramethyl -3,4,5,6,7,8-hexahydro-2H-3a,7-methanoazulen-2-one (6.71%), 13-methyl-Z-14-nonacosene (1.08), 5(1H)-azulenone (34.67%), guaia-1(10), 11-diene (9.54%), γ-elemene (6.59%), 11-octadecenoic acid methyl ester (5.97%), hexadecanoic acid methyl ester (5.95%), trans-13-octadecenoic acid (5.01%), 1-ethoxy-2,2-dimethyl-3-(2-phenyl ethynyl)-cyclopropane (4.10%),  9(1H)-phenanthrone (3.28%), longifolene (2.93%), 2-hydroxycyclopentadecanone (2.92%), patchoulene (2.59%), 9-octadecenoic acid methyl ester (1.72%), oleic acid (1.26%) and 2-octylcyclopropaneoctanal (1.10%). The present study indicates that the stem and leaf of J. secunda Vahl is rich in volatile compounds that can provide useful medicinal or other industrial purposes.


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Author Biography

Edet O. Odokwo, Federal University of Otuoke, Bayelsa State, Nigeria

Department of Chemistry


Abo, K.J.C., Kouakou, K.L., & Yapo, A. (2016). Hypotensive and hypertensive effects of total aqueous extract of Justicia secunda Vahl M. (Acanthaceae) in rabbits. Intern J Sci Res, 5, 5, pp. 1455-1462.

Aikoye, A. O. (2020). Theoretical and biochemical information studies on compounds detected in GCMS of ethanol extract of Chromolaena odorate. Communication in Physical Sciences, 6, 1, pp. 635-648.

Arogbodo, J.O. (2020). Evaluation of the Phytochemical, proximate and elemental constituents of Justicia secunda Vahl M. International Journal of Innovative Science and Research Technology. 5, 5, pp. 1262-1268.

Calderon, A.I., Hodel, A., Wolfender, J., Gupta, M.P., Correa, M., & Hostettmann, K. (2013). LC-DAD-MS-based metabolite profiling of three species of Justicia (Acanthaceae). NatProd Res, 27, 15, pp. 1335-42.

Carrington, S., Cohall, D.H., Gossell-Williams, M., & Lindo, J.F. (2012).The antimicrobial screening of a Barbadian medicinal plant with indications for use in the treatment of diabetic wound infections. West Ind Med J, 61, 9, pp. 861-864.

Chibuzo, O. U. & Aikoye, A. O. (2020). Chemical Information from GCMS analysis of acetoneethanol extract of Piper guineense leaf. Part 2. Communication in Physical Sciences, 5(4): 670- 481.

Chibuzo, O. U. & Okop, I. (2020). Chemical Information from GCMS analysis of acetone extract of Piper guineense leaves. Part 1. Communication in Physical Sciences, 5(4): 437- 445.

Chifundera, K. (2001). Contribution to the inventory of medicinal plants from the Bushi area, South Kivu Province, Democratic Republic of Congo. Fitoterapia, 72, 4, pp. 351-368.

Corrȇa, G.M., & Alcȃntara, A.F. (2012). Chemical constituents and biological activities of species of Justicia- A Review. Braz. J. Pharmacogn., 22, 1, pp. 220-238.

Eddy, N. O,,Awe, F. E., Siaka, A., Magaji, L. & Ebenso, E. E. (2011).Chemical information from GC-MS studies of ethanol extract of Andrographis paniculata and their corrosion inhibition potentials on mild steel in HCl solution. International Journal of Electrochemical Sciences, 6, pp. 4316-4328.

Eddy, N. O. (2010). Adsorption and inhibitive properties of ethanol extract of Garcinia kola and Cola nitida for the corrosion of mild steel in H2SO4. Pigment and Resin Technology, 39, 6, pp. 347-353.

Eddy, N. O., Awe, F. & Ebenso, E. E. (2010). Adsorption and inhibitive properties of ethanol extracts of leaves of Solanum melongena for the corrosion of mild steel in 0.1M HCl. International Journal of Electrochemical Science, 5, pp. 1996-2011.

Eddy, N. O., Odiongenyi, A. O., Ameh, P. O. & Ebenso, E, E, (2012). Corrosion inhibition potential of Daniella oliverri gum exudate for mild steel in acidic medium. International Journal of Electrochemical Sciences, 7, pp.7425-7439.

Hamiltons-Amachree, A., & Uzoekwe, S.A. (2017). GC-MS analysis of oil rich in polyenoic fatty acid methyl esters from leaves of Justicia secunda Vahl growing abundantly in the lowland rain forests of the Niger Delta region of Nigeria. American Journal of Essential Oils and Natural Products, 5, 4. Pp. 1-4.

Herrera, M.H., Rosas, R.A., & Crescente, O.V. (2012). Biological activity of “Sanguinaria” (Justicia secunda) extracts. Pharmaceutical Biology, 40, 3, pp. 206-212.

Ikpeazu, V. O., Otuokere, I. E. & Igwe, K. K. (2020). Gas Chromatography–Mass Spectrometric analysis of bioactive compounds present in ethanol extract of Combretum hispidum (Laws) (Combretaceae) root. Communication in Physical Sciences, 5, 3, pp. 325-337

Koffi, E.N., Le Guernevé, C., Lozanoa, P.R., Meudec, E., Adjé, F.A., Bekro, Y., & Lozano, Y.F. (2013). Polyphenol extraction and characterization of Justicia secumda Vahl leaves for traditional medicinal uses. Ind Crops Prod, 49: pp. 682-689.

Koné, W.M., Koffi, A.G., Bomisso, E.L., & TraBi, F.H. (2012). Ethnomedical study and iron content of some medicinal herbs used in traditional medicine in Cote D’ivoire for the treatment of anaemia. Afr J Tradit Complement Altern Med, 9, 1, pp. 81-87.

Lans, C. (2007). Ethnomedicines used in Trinidad and Tobago for reproductive problems. J Ethnobiol Ethnomed, 15, pp. 3-13.

Lee, M. O. & Choi, J.C. (2012). “Aromatheraphy for health care: an overview of systemic reviews”. Maturitas, 3, 71, pp. 257-260.

Li, X., Wang, G., Zhao, J., Ding, H., Cunningham, C., Chen, F., Flynn, D. C., Reed, E.& Li, Q. Q, (2005). Antiproliferative effect of beta-elemene in chemoresistant ovarian carcinoma cells is mediated through arrest of the cell cycle at the G2-M phase. Cellular and Molecular Life Sciences. 62, 7,8, pp. 894-904.

Liu, M. H., Zhang, Q., Zhang, Y. H., Fu, X. Y. Fu, W. M. & Hi, J. Y. (2016). Chemical aanalysis of ddietary cconstituents in Rosa roxburghii and Rosa sterilis fruits. Molecules, doiI: 10.3390/molecules21091204.

Manda, P., Abrogoua, D.P., Bahi, C., Dano, D.S., Gnaboui, G., & Kablan, B.J. (2011). Evaluation of the antihypertensive activity of total aqueous extract of Justicia secunda Vahl (Acanthaceae). Afric J Pharm Pharmacol, 5, 16, pp. 1838-1845.

Moswa, J.L., Kapanda, N., Mungende, D.M., Okitolonda, W., Mayangi, M., Mihigo, S., & Mbale K. (2005). Plant as an important source of iron for the treatment of Anaemia: Case of Justicia secunda. 11th NAPRECA Symposium Book of Proceedings, Antananarivo, Madagascar, pp. 132-135.

Mpiana, P.T., Bokota, M.T., Ndjele, M.B.L, Mudogo, V., Tshibangu, K.N., Ngbolua, K.N., Atibu, E.K., Kwembe, J.T.K., & Makelele, L.K. (2010). Antisickling activity of three species, Antisickling activity of three species of Justicia from Kisangani (DR Congo): J. tenella, J.gendarussa and J. insularis. Int J Biol Chem Sci, 4, 6, pp. 1953-1961.

Mpiana, P.T., Ngbolua, K.N.N., Bokota, M.T., Kasonga, T.K., Atibu, D.S.T., & Mudogo, V. (2010). In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfus, 8, pp. 248-254.

Musa, R, (2020b). Proximate composition of leaf and phytochemical screening of leaf, stem and root of Tridax procumbens cultivated in North-East Nigeria. Communication in Physical Sciences, 5, 4, pp. 606-610

N’guessan, K., Kouassi, K.H., & Ouattara, D. (2010). Plants used to treat anaemia, in traditional medicine, by Abbey and Krobou populations, in the South of Côte-d’Ivoire. J Appl Sci Res, 6, pp, 1291-1297.

Ogoko, E. C. (2020). Chemical information from GCMS of ethanol extract of Solanum melongena (aubergine) leaf. Communication in Physical Sciences, 6, 1, pp. 658-666.

Okwunodulu, F. U. &Eddy, N. O. (2014). Equilibrium and thermodynamic consideration of Cd2+, Ni2+ and Pb2+ removal from aqueous solution onto treated and untreated Cola nitida waste biomass. International Journal of Science and Research (IJSR), 2(, 3, pp. 567-569.

Onoja, S.O., Ezeja, M.I., Omeh, Y.N., & Onwuke, B.C. (2017). Antioxidant, anti-inflammatory and antinoceptive activities of methanol extract of Justicia secunda Vahl leaf. Alexandria Journal of Medicine, 53, pp. 207-213.

Onyeabo, C., Achi, N.K., Ekeleme-Egedigwe, C.A., Ebere, C.U., & Okoro, C.K. (2017). Haemotological and biochemical studies on Justicia carnea in albino rats. Acta Sci. Pol. Technol. Aliment., 16, 2, pp. 217-230.

Oruk, A. E., Ibuot, A. A. & Echeme, J. O. (2020). Comparative studies of the secondary metabolites in the root and leaf of Starchytarpheta cayennennsis (L) Vahl. Communication in Physical Sciences, 5, 1, pp. 46- 50.

Osioma, E. & Hamilton-Amachree, A. (2017). Comparative study on the Phytochemical and in vitro antioxidant properties of methanolic leaf extract of Justicia secunda Vahl. Nigerian Journal of Science and Environment, 15, 1, pp. 111-117.

Rojas, J.J., Ochoa, V.J., Ocampo, S.A., & Munoz, J.F. (2006). Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: a possible alternative in the treatment of non-nosocomial infections. BMC Compl Alter Med., 17, pp. 6-12.

Runde, M. (2020a). Preliminary screening of medicinal plants in Nigeria for phytochemicals and essential oil constituents. Communication in Physical Sciences, 5(4): 669-674.

Sitarek, P., Rijo, P., Garcia, C., Skala, E., Kalemba, D., Bialas, A. J., Szemraj, J., Pytel, D., Toma, M., Wysokinska, H. and Sliwinski, T. (2017). Antibacterial, anti-Inflammatory, antioxidant, and antiproliferative properties of essential oils from hairy and normal roots of Leonurus sibiricus L. and their chemical composition, Oxidative Medicine and Cellular Longevity, 2017, dx.doi.org/10.1155/2017/738406.

Sivapalan, S. R. (2013). Medicinal uses and pharmacological activities of Cyperis rotundus Linn- A review. International Journal of Scientific and Research Publications, 3, 6, pp. 1-7.

Theiler, B.A., Revoltella, S., Zehl, M., Dangl, C., Caisa, L.O.E., König, J., Winkler, J., Urban, E., & Glasl, S. (2014). Secundarellone A, B and C from the leaves of Justicia secunda VAHL. Phytochem Lett, 10: pp. cxxix-cxxxii.

Yao, Y. Q., Ding, X., Jia, Y. C., Huang, C. X, Wang, Y. Z., Xu, Y. H. (2008). Antitumor effect of beta-elemene in glioblastoma cells depends on p38 MAPK activation. Cancer Letters, 264, 1, pp. 127–134.

Zhu, T., Xu, Y., Dong, B., Zhang, J., Wei, Z., Xu, Y. & Yao, Y. (2011). Β-elemene inhibits proliferation of human glioblastoma cells through the activation of glia maturation factor β and induces sensitization to cisplatin. Oncology Reports, 20, 2, pp. 405-41326 (2): 405–13.