Isolation and Characterization of Stigmasterol and Β-Sitosterol from the leaves of Emilia coccinea (Sims) G.Don
Keywords:Emilia coccinea, extraction, stigmasterol and β-sitosterol, characterization
Communication in Physical Science 2020, 6(2):883-868
Authors: Ugochukwu Clinton Akwada*, Okenwa Uchenna Igwe, Friday Chisom and Juliet Chiamaka Ibe - Diala
Received 27 November 2020/Accepted 27 December 2020
In view of its unique medicinal value and other applications, stigmasterol and β-sitosterol were isolated from Emilia coccinea leaves. Extraction of the compounds from the plant was carried out using Soxhlet apparatus, while column and thin layer chromatographic techniques were used for isolation and purification, which afforded white crystalline powder. Structural elucidation using 1H-NMR, DEPT, COSY, HSQC, and HMBC followed by comparison, with literature values confirmed the isolated compounds as stigmasterol and β-sitosterol. The isolated compounds may contribute to the medicinal properties of E. coccinea. This is the first isolation of stigmasterol and β-sitosterol from E. coccinea.
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