Phytochemical screening and wound healing studies of Chromolaena odorata

Abstract

Chromolaena odorata (Siam weed), traditionally used for wound healing, possesses a rich profile of bioactive compounds. In this study, Chloromolaena odorata s investigated for its phytochemical and pharmacological properties. The results arising from phytochemical screening of the leaf extract validated the presence of cardiac glycosides (2.63 mg/100 g), flavonoids (4.90 mg/100 g), terpenoids (5.44 mg/100 g), alkaloids (7.56 mg/100 g), tannins (9.20 mg/100 g), and saponins (11.8 mg/100 g). Methanol crude extract of the phytochemicals obtained from the plant leaf was also used in running a column chromatography. Toxicity evaluation and dermal irritation of the methanol leaf extract of C. odorata verifies that it is non-toxic upto 5000 mg/kg. Two purified fraction of the methanol crude obtained from column chromatography and the methanol crude extract were applied for conducting wound healing tests and the test results gave a significant evidence that the extract has a wound healing property through its activity to decrease both bleeding and clothing time, which could be as a result of the increased in platlet count.. The results suggest that Chromolaena odorata plant can be exploited in as a source of traditional medicine.