Proximate Analysis, Thin Layer Chromatography Profile and Haematinic Activity of Organic Extracts of Brillantaisia Owariensis Leaves

Abstract

Onuchi. M. Mac-kalunta*,  Ahamefula. A Ahuchaogu and*Johnbull O Echeme

In this work, the proximate analysis, concentration profile from thin-layer chromatography, and haematinic activity in rats induced phenylhydrazine anemia were investigated using the n-hexane, chloroform, ethyl acetate, and methanol extracts of Brillataisia owariensis leaves. The results we recorded indicated a significant presence of carbohydrates, fiber, protein, and traces concentrations of fat. Analysis of the thin layer chromatogram gave evidence that the plant extract is rich in various phytochemicals. However, the activity of the plant extract based on the hematological parameters (red blood cell count (RBC), hemoglobin concentration (HB), white blood cell count (WBC), and hematocrit (PCV), showed that the ethyl acetate extract has the least activity, whereas, the crude methanol and chloroform extracts demonstrated the most significant activity.