Regulatory Gene, aflaR Identification of Aflatoxigenic Moulds and total Aflatoxin estimation in Digitaria exilis and Digitaria iburua sold within Kaduna Metropolis
Keywords:Aflatoxigenic moulds, aflaR, aflatoxins, carcinogen, Digitaria sp
Communication in Physical Sciences, 2023, 9(1):25-33
Authors: Joseph Reuben Wartu., Sani Sambo Datsuquai Mohammed., John Idakwoji and Tessy Bamai
Received: 26 November 2022/Accepted 25 February 2023/
Contamination of Digitaria sp by aflatoxigenic strains could pose a serious health threat to food safety. In this study, a design is made with the aim of screening and characterizingmoulds isolated from Digitaria iburua and Digitaria exilis sold within the Kaduna metropolis. Seventy samples of Digitaria sp were obtained from four outlets of some markets within the Kaduna metropolis. The proximate values were determined following standard procedures. Isolation and characterization of the moulds were done using standard techniques. Polymorphic technique and regulatory gene aflaR were employed to identify the aflatoxigenic moulds isolated from the samples. Analysis of the aflatoxin content of the samples was carried out using Enzyme-Linked Immunosorbent Assay (ELISA) technique. This research has recorded proximate values determined for Digitaria sp as follows: moisture content 6.90%, protein 11.10 %, ash 3.90 %, fats 3.9 %, and carbohydrate content 74.16 % for Digitaria iburua. Digitaria exilis presented a moisture content of 7.3 % while other proximate contents were protein (10.2%), ash (3.21%), fats (2.3%), and carbohydrate content (76.99%) %. The moulds; Aspergillus flavus, Rhizopus stolinifer, Fusarium sp, Saccharomyces cerevisae, Aspergillus parasiticus, Aspergillus niger, mucor sp and Penicillium sp were isolated from both Digitaria exilis and Digitaria exilis with Aspergillus flavus having the highest frequency of occurrence. The total viable mould count for each sample location ranged from 1.5X103 – 2.8X103 CFU/g, 2.3X103-3.1X103 CFU/g, 2.6X103-5.4X103 for Kawo, Sabo and Central markets respectively. Analysis of the total aflatoxin content of the seventy samples showed a prevalence of 16.7 %. There is a need to sensitize farmers and consumers on the occurrence of the toxin and also the need to develop a resistant variety is hereby advocated.
Archana Sawane & Manish Sawane (2014). Mycotoxigenicity of Aspergillus, Penicillium and Fusarium spp. Isolated from stored rice. International Journal Current Microbiology and Applied Science, 3, 11, pp. 116 -121
Association of Official Analytical Chemists, AOAC (1984). Official methods of analysis (14th edition) of AOAC International. Washington, DC, USA. Official Method, 14.022
Bankole, S.A., and Adebanjo, A. (2003). Mycotoxins in food in West Africa: current situation and possibilities of controlling it. African Journal of Biotechnology, 2, pp. 254–263.
Bankole, S. A., Schollenberger, M., & Drochner, W. (2006). Mycotoxins in food systems in sub Saharan Africa: a review. Mycotoxin Research, 22, pp. 163–169.
Bullerman, M. O. & Andrea, I. A. (2011). Traditional and possible technological uses of Digitaria exilis (finio). Plant Foods for Human Nutrition, 54, pp. 363-374.
Cotty, P. .J. (1994). Influence of field application of an atoxigenic strain of Aspergillus flavus on the populations of A. flavus infecting cotton bolls and on the aflatoxin content of cottonseed. Journal of Pathology, 84, pp. 1270–1277.
Cruz, J. F. (2004). Fonio: a small grain with potential. MLEISA. 20, pp. 16–17.
David, S. O. & Jones, D.W. (2008). Isolation and physiochemical Characterization of Digitaria sexilis. 49, pp. 131-135.
Donner, M., Atehnkeng, J., Sikora, R.A., Bandyopadhyay, R. & Cotty, P.J. (2009). Distribution of Aspergillus section Flavi in soils of maize in three agroecological zones of Nigeria. Soil Biology and Biochemistry. 41, pp. 37–44.
European Commission (2006). Commission Regulation No. 1881/2006, setting maximum levels of certain contaminants in foodstuffs. Official Journal of the European Union, L364/5
Ezekiel, C.N., Sulyok, M., Warth, B. & Karka, R. (2012). Multi-microbial metabolites in fonio millet (acha) and sesame seeds in Plateau State, Nigeria. European Food Research Technologgy. 235, pp. 285–293.
Gbodi, T.A., Nwude, N., Aliu, Y.O., and Ikediobi, O. (2004). The mycoflora and some mycotoxins found in acha (Digitaria exilis stapf) in PlateauState, Nigeria. Food Chemistry Toxicology. 24, pp. 339–342.
[IARC] International agency for research on cancer (1993). Aflatoxins some naturally occurring substances: Food items and constituents, heterocyclic amines and mycotoxins. IARC scientific Publications.IARC, Lyon. 56.
Klich, M. A. (2002). Identiﬁcation of common Aspergillus species. Central bureau Schimmelcultures, 5, pp. 143- 147.
Larry R. Beuchat (1983).Influence of Water Activity on Growth, Metabolic Activities and Survival of Yeasts and Molds. Journal of Food Protection, 46, 2, pp. 135 141
Nzelibe, H. C., Obaleye, S. & Onyenekwe, P. C. (2000). Malting characteristics of different varieties of fonio millet (Digitaria exilis). European Food Research Technology. 211, pp. 126-129.
Ordaz, J. Jaimez., C.A. Fente, B.I. Va´zquez, C. M. Franco, A. & Cepeda, (2003) Development of a method for direct visual determination of aflatoxin production by colonies of the Aspergillus flavus group. International Journal of Food Microbiology, 83, pp. 219–225.
Ogbonnaya Chukwu (2009). Physicochemical properties of acha (Digitaria exilis and Digitaria iburua spp) grains. Int. J. Postharvest Technology and Innovation,1, 4, pp. :359-366
Scheidegger, K.A. and Payne, G.A., 2012. Unlocking the secrets behind secondary metabolism: A review ofAspergillus flavus from pathogenicity to functional genomics. Journal of Toxicology, 22, pp. 423-459.
Scherm,B.,Palomba,M.,Serra,O.,Marcello, & Migheli, Q.(2005). Detection of transcripts of the aflatoxin genes aflD, aflO, and aflP by reverse transcription-polymerase chain reaction allows differentiation of aflatoxin-producing and non-producing. Journal of Food Microbiology, 98, pp.201-210.
Smith, J. E. and Moss, M. O. (1985). Mycotoxins. Formation, analysis and significance. John Wiley and Sons Ltd., ISBN 0-471-90671-9, Chichester.
Strosnidere, R., Simmonds, D. H. & O'Brien, T.P. (2010). Morphological and biochemical development of wheat endosperm. Advance Cereal Science Technology. 4, pp. 5-17.
Somorin, Y. M. & Bankole, S. A. (2010). Mycoflora of stored “Ofada” and “Abakaliki” rice in Lagos and Ogun States, Southwestern Nigeria. African Journal of Microbiology Research. 4, 1-6, pp. 1724-1726.
Umoh, N. J., Lesi, O. A., Mendy, M., Bah, E., Akano, A., Whittle, H., Hainaut, P. & Kirk, G. D. (2011). Aetiological differences in demographical, clinical and pathological characteristics of hepatocellular carcinoma in The Gambia. Liver International 31, pp. 215- 221
Wartu, J.R., Whong, C.M.Z., Abdullahi, I.O. and Ameh, J.B. and Diya, A.W. (2015). Occurrence of aflatoxin levels in harvest and stored groundnut kernels in Kaduna state, Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology, (IOSR- JESTFT). 9, 1, pp. 62-66.
Wartu, J.R., Whong, C.M.Z., Abdullahi, I.O. and Ameh, J.B. (2017). Phylogenetics of Aflatoxigenic Moulds and Prevalence of Aflatoxin From In-Process Wheat and Flour from Selected Major Stores Within Northern Nigeria. Science World Journal, 12, pp.4:83 - 87
Wartu J. R.(2019).Mycology And Effects of Dehauling Local Oryza Sativa For Human Consumption on Total Aflatoxins Within Some Parts of Kaduna Metropolis. Science World Journal, 14, 2, pp. 89 92
Williams, J. H., Phillips, T. D., Jolly, P. E., Stiles, J.K., Jolly, C.M., Aggarwal, D.(2004). Human aflatoxicosis in developing countries: A review of toxicology, exposure, potential health consequences, and interventions. American Journal of Clinical Nutrition, 80, pp1106 –1122.
Wild, C.P. (2007). Aflatoxin exposure in developing countries: the critical interface of agriculture and health. Food Nutrition. 28, PP. 372-380.
Yu, J., Chang, P.K., Ehrlich, K.C., Cary, J.W., Bhatnagar, D., Cleveland, T.E., Payne, G.A., Linz, J.E., Woloshuk, C.P. & Bennett, J.W. (2004). Clustered pathway genes in aflatoxin biosynthesis. Appl. Environ. Microbiol. 70, PP. 1253-1262.
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