Phytoconstitution and Antimicrobial Activity of Costus Lucanusianus Floral Volatile Extract
Keywords:
Phytochemical constituents, volatile components, plant extracts, Costus lucanusianus, antimicrobial activityAbstract
Communication in Physical Sciences, 2023, 9(1):73 -79
Authors: Akens Hamilton-Amachree and Ngozi M. Uzoekwe
Received: 12 December 2022/Accepted 30 March 2023
Information on volatile constituents of plant extracts have a significant backing to theseveral biological activities and applications of plants. Consequently, in this work, phyto content and the antimicrobial activity of the volatile floral extract of Costus lucanusianus are investigated in this work to complement the information database on plant volatile components. The volatile constituents of the floral part were extracted using the conventional hydrodistillation technique and its phyto-constitution was determined via Gas Chromatography-Mass Spectrometry (GCMS) instrumentation. The antimicrobial pharmacological activity was established using the pour method against eight (8) strains of microbes. The volatile extract has a total of twenty-three (23) phytochemicals comprising eight (8) sesquiterpenes, four (4) sesquiterpenoids and eleven (11) non-terpenoids. The major identified constituents are 4-(3-hydroxy-2-methoxyphenyl)-but-2-one (38.29%), 1-(4-Hydroxymethoxy-phenyl)dec-4-en-3-one (13.83%),[S-(R*,S*)] 5-(1,5-dimethyl-4-hexenyl)-2-methyl-1,3-cyclohexad- iene (7.66%), 1-(1,5-dimethyl-4-hexenyl)-4-methyl- benzene (6.13%), decanal (4.35%),[S-(R*,S*)]-3-(1,5-dimethyl-4-hexenyl)-6-methy- lene- cyclohexene (3.47%) and β-bisabolene (2.76%). The antimicrobial activity witnessed a dose-dependent gradient extract–inhibitory relationship. The highest inhibitory activity was observed at 100 mg/ml for all the strains. There was no inhibition observed below 12.5 mg/ml for Escherichia coli and Fusarium spp.
while Staphylococcus aureus, Bacillus subtillis, Pseudomonas aeruginosa, Salmonella typhi, Candida albicanandKlebsiela pneumonia showed no inhibition below 6.25 mg/ml. The present study has established that the floral part of Costus lucanusianus has volatile constituents with antimicrobial potency
Downloads
References
James, D., Kantar, D., Kreft, S. & Prosen, H. (2008). Identification of buckwheat (fagopyrum esculentum Moench) aroma compounds with GC-MS. Food Chemistry, 112, pp. 120-124.
Nurzynska-Wierdak, R. (2013). Essential oil composition of the coriander (Coriandum sativum L.) herb depends on the development stage. Acta Agrobotanica, 66, pp.53-60.
Al-Marzoqi, H., Hadi, M.Y. &Hameid, I.H. (2016). Determination of metabolites products by Cassia angustifolia and evaluate antimicrobial activity. Journal of Pharmacognosy and Phytotherapy, 8,2, pp. 25-48.
Maurya, V.K., Kumar, S., Prasad, A.K., Bhatt, M.L.B. & Saxena, S.K. (2020). Structure-based drug designing for potential antiviral activity of selected natural products from Ayurveda against SARS-CoV-2 spike glycoprotein and its cellular receptor (2020). Virus Disease, 31, pp. 179-193.
Spakowicz, D.J. & Strobel, S.A. (2015). Biosynthesis of hydrocarbons and volatile organic compounds by fungi: Bioengineering potential. Applied Microbiologyand Biotechnology, 29, 12, pp. 4943-4951.
Lu, F. & Teal, P.E. (2001). Sex pheromone components in oral secretions and crop of male Caribbean fruit flies, Anastrepha suspense (Loew). Archives of Insect Biochemistry and Physiology, 48,3, pp. 144-154.
Chen, C., Wang, J., Sun, M., Li, J. and Wang, H.D. (2022). Toward the next-generation phyto-nanomedicines: cell-derived nanovesicles (CDNs) for natural product delivery. Biomedicine and Pharmacotherapy, 145, pp. 112416
Ashraf, S.A., Al-Shammari, E., Hussain, T., Tajuddin, S. & Prasad, B. (2017). In vitro antimicrobial activity and identification of bioactive components using GC-MS of commercially available essential oils in Saudi Arabia. Journal of Food Science and Technology, 54, pp. 3948-3958.
Shareef, H., Haidar, J., Hussein, H.M. & Hammeed, I.H. (2016). Antibacterial effect of ginger (Zingiber officinale) Roscoe and bioactive chemical analysis using gas chromatography mass spectrum. Oriental Journal of Chemistry, 32, 2, pp. 817-837.
Eddy, N.O. (2010). Adsorption and inhibitive properties of ethanol extract of Garcinia kola and Cola nitida for the corrosion of mild steel in H2SO4. Pigment and Resin Technology, 39, 6, pp. 347-353.
Peters, D.E. & Chibueze, I. (2022a). Nutrient’s composition of Costuslucanusianusstem. GSC Biological and Pharmaceutical Sciences, 18, 2, pp. 70-81.
Peters, D.E. & Chibueze, I. (2022b). Qualitative phytochemicals and essential oil constituents of Costuslucanusianusstem. GSC Biological and Pharmaceutical Sciences, 18, 2, pp. 82-97.
Chan, W., Tan, L.T., Chan, K., Lee, L & Goh, B. (2016). Nerolidol: A sesquiterpene alcohol with multi-faceted pharmacological and biological activities. Molecules, 21, 5, pp. 529.
Aparna, V., Dileep, K.V., Mandal, P.K., Karthe, P., Sadasivan, C. & Haridas, M. (2012). Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chem Biol Drug Des., 80, 3, pp. 434-439.
Chen, C., Liu, T., Liu, Y. & Tseng, W. (2007). 6-Shogaol (alkanone from ginger) induces apoptotic cell death of human hepatoma p53 mutant mahlavu subline via an oxidative stress-mediated caspase-dependent mechanism. Journal of Agricultural and Food Chemistry, 55, 3, pp. 948-954.
Hamilton-Amachree, A. & Odokwo, O.E. (2022). GC-MS analysis of the volatile activity of the crude honey residue from Takum Local Government Area of Taraba State, Nigeria. African Scientist, 23, 3, pp. 193-199.
Odokwo, E.O. & Onifade, M.S. (2020). Volatile constituents of the leaves and stem of Justicia secunda Vahl. Communication in Physical Sciences, 6, 2, pp. 827- 834.
Odokwo, E.O. & Salawu, R.A. (2021). Chemical and biological profiling of n-hexane extract of crude honeybee residue. World Scientific News, 152, pp. 69- 81.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 The Journal and the author
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
