Evaluation of Antioxidant Activity of Ethanol Extract of Root and Stem Bark of Moringa oleifera (MO) obtained from Utu Ikpe, Ikot Ekpene Local Government Area, Nigeria
Keywords:
Moringa oleifera, phytochemicals, free radical scavenging activity, free radical assay and reducing powerAbstract
Communication in Physical Sciences, 2022, 8(1): 1-8
Authors: Patrick. G. Udofia*, Philippa. C. Ojimelukwe, A. O. Olaoye, Anthony. N. Ukom, Moses L. Ekanem and I. I. Okparauka
Received: 26 November 2021/Accepted 20 February 2022
The work studied free radical scavenging activities of ethanol extract of the stem bark and root bark of MO. They are used by the local population to manage common health problems. Antioxidant activities of the plant parts extracts were studied spectrophotometrically using DPPH (1,1-dimethyl-2-picrylhydrazyl radical), (FRAP) ferric reducing antioxidant power, (H2O2) hydrogen peroxide, ABTS (2,2’azinobis (3-ethylbenzthiozoline-6-sulphonic acid), (NO) nitric oxide as basic free radicals. The result of phytochemical screening and quantitative content of the plant parts revealed a yield of (2.84%) and 4.34% for the stem and root barks respectively. Alkaloids (2.47%), saponin (0.169%), tannins (3.35%), flavonoids (1.34%) and Alkaloids (1.01%), saponin (0.03%) tannins (1.87%), flavonoids (traces) were estimated in the stem bark and root bark respectively. The total phenolic contents in the stem and root barks were (25.21 GAE/g) and (13.37 RE/g) of the dry samples respectively. The stem and root barks of the samples showed 5.30 AAE/g and 4.90 AAE/g respectively. Models of extracts on the basic free radicals were significant (p<0.05; R2>90%) and acted linearly as a function of the phytochemical content and the free radicals’ models in use. The IC50 of the stem bark and root bark on FRAP was 21.41±0.01 and 59.20±0.11 AAE/g respectively compared with ascorbic acid (40.56). The result indicated that ethanol extract of the plant parts may constitute a good source of natural compounds with antioxidant capability in the food and general health sector
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References
Abalaka, M. E., Daniyan, S. Y., Oyeleke, S. B. & Adeyemo, S. O. (2012). The antibacterial evaluation of M. oleifera leaf extracts on selected bacterial pathogens. Journal of Microbiology Research, 1, pp. 1-4.
Abalaka, M. E., Olonitola, O. S. & Onaolapo, J. A. (2009). Evaluation of acute toxicity of Momordiac charantia extracts, using Wistar rats to determine safety level and usefulness of the plant ethnotherapy. International Journal Applied Science i, 3:1-6.
Embaby, H. S. & Mokhtar, S. M. (2011) Chemical composition and nutritive value of lantana and sweet pepper seeds and Babak seed kernels. Journal of Food Science, 76, 5, pp. 736-742. doi: 10.1111/j.1750-3841.2011.02166.x.
El-Sohaimy, S. A., Hamad, G. M., Mohamed, S. E., Amar, M. H. & Al-Hindi, R. R. (2015). Biochemical and functional properties of Moringa oleifera leaf and their potential as a functional food. Global Advanced Research Journal of Agricultural Science, 4, 4, pp. 188-199.
Fahey J. W. (2005). M. oleifera: A Review of the Medical Evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal 1:5.
Garima, M., Pradeep, S., Ramesh, V., Sunil, K., Saurabh, S., Jha, K. K. & Khosa, R. L. (2011). Traditional Uses, phytochemistry and pharmacological properties of Moringa oleifera plant: An overview. Der PharmaciaLettre, 3, 2, pp. 141-164.
Gordon, M. H. (1990) The mechanism of anti-oxidant action in vitro. In: BJF Hudson (Ed.) Food antioxidant Elsevier Applied Science, London, pp. 1-8.
Kumari, K. L., N. W., Abeysinghe, D. C. & Dharmadasa, R. M. (2016,) Distribution of phytochemicals and bioactivity in different parts and leaf positions Stevia rebaudiana of (bertoni) bertoni- a non-caloric, natural sweetener. World Journal of Agricultural Research, 4, 6, pp. 162-165. DOI:10.12691/wjar-4-6-2
Capsimum annuum cultivars during fruit development., LWT – Food Science and Technology, 53, 1, pp. 370-377.
Ujah, O. F.,, Mohamma, Y. Y. & Dewua, C. M. (2012). Quantification, antioxidant and free radical scavenging potentials of polyphenols from crude extracts of Phyllanthus amarus leaves. ChemSearch Journal, 12, 1, pp. 91-107.
Nishtha, K., , P., Nistin, M. & Ram, P. S. (2012). Antibacterial and antioxidant activity of different extracts of M. oleifera leaf in in vitro study. International Journal of Pharmaceutical Sciences Review and Journal of Agriculture and Food Chemistry, Research, 12, 1, pp. 112- 124.
Kumar, V., Pandey, N., Mohan, N. & Singh, R. P. (2012). Antibacterial and antioxidant activity of different extracts of M. oleifera leaf in vitro study. International Journal of Pharmaceutical Sciences Review and Research, 12, 1, pp. 89-94.
Yen, G. C. & Chen, H. Y. (1995) Antioxidant activity of various tea extracts in relation to their anti-mutagenic, Journal of Agricultural and Food Chemistry, 43, pp. 27-37.
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