Phytochemical Screening, GC-MS And FTIR Analysis of Ethanol Extract of Piliostigma thonningii (schum Milne—Redth) Leaf

Abstract

Authors: Isah Yinusa and Joy Oremeyi Raphael

The present investigation was carried out to investigate the phytoconstituent of piliostigma thonningii leaf through phytochemical screening GC-MS analysis and Fourier Transform Infrared Spectroscopy (FTIR). Cold extraction method was used to extract the organic constituent of the plant leaf using ethanol as a solvent. The result obtained indicated a yield of 4.6 g (3.06 %). Preliminary phytochemical screening revealed the presence of flavonoid, terpenoid, cardiac glycosides, tannins, phytosterols, phlobatannins and alkaloid. The GC-MS analyses of ethanol leaf extracts showed the presence of 64 components based on separation of individual peaks through the column with respect to retention time (Rt) and area under the respective peaks. The prominent molecular functional vibration of chemical groups was also determined. The peak at 3250.2 cm-1 was assigned to hydroxyl vibration in alcohol while the band at 2918.5cm^-1, 2847.7 cm-¹.  and 142.1cm^-1 were attributed to the prescence of alkanes.