Phytochemical screening and wound healing studies of Chromolaena odorata
Keywords:
Chloromolaena odorata, phytochemical, wound healing, column chromatographyAbstract
Communication in Physical Sciences, 2024, 11(2): 265-275
Authors: Nwoke Linda Chinyere*, Okenwa Uchenna Igwe, Onuchi Marygem Mac-Kalunta and Johnbull Onyekachi Echeme, Uyanwa Maryjane Nkeiruka
Received: 12 July 2023/Accepted: 29 April 2024
Chromolaena odorata (Siam weed), traditionally used for wound healing, possesses a rich profile of bioactive compounds. In this study, Chloromolaena odorata s investigated for its phytochemical and pharmacological properties. The results arising from phytochemical screening of the leaf extract validated the presence of cardiac glycosides (2.63 mg/100 g), flavonoids (4.90 mg/100 g), terpenoids (5.44 mg/100 g), alkaloids (7.56 mg/100 g), tannins (9.20 mg/100 g), and saponins (11.8 mg/100 g). Methanol crude extract of the phytochemicals obtained from the plant leaf was also used in running a column chromatography. Toxicity evaluation and dermal irritation of the methanol leaf extract of C. odorata verifies that it is non-toxic upto 5000 mg/kg. Two purified fraction of the methanol crude obtained from column chromatography and the methanol crude extract were applied for conducting wound healing tests and the test results gave a significant evidence that the extract has a wound healing property through its activity to decrease both bleeding and clothing time, which could be as a result of the increased in platlet count.. The results suggest that Chromolaena odorata plant can be exploited in as a source of traditional medicine.
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Azebaze, A. G. B., Meyer, M., Valentin, A., Nguemfo, E L., Fomum, Z.T.& Nkengfack, A.E. (2006). Prenylated xanthone derivatives with antiplasmodial activity from Allanblackia monticola STANER LC. Chem Pharm Bull. 54, 1, pp. 111–113.
Aziz, N. A., Farouk, A. A., & Yusof, M. I. (2018). The Pharmacological Properties and Medicinal Potential of Chromolaena odorata: A Review. Evidence-Based Complementary and Alternative Medicine, 2018, pp. 1–13. DOI: 10.1155/2018/9272682.
Aziz, N. A., Farouk, A. A., & Yusof, M. I. (2018). The Pharmacological Properties and Medicinal Potential of Chromolaena odorata: A Review. Evidence-Based Complementary and Alternative Medicine, 2018, 1–13. DOI: 10.1155/2018/9272682
Bamikole, M.A., Ikhatua U.J. & Osemwenkhae A.E. (2004).Converting bush to meat: A case of Chromolaena odorata feeding to rabbits. Pakistan Journal of Nutrition. 3, pp.258–261.
Brazilian J. of Microbiology,2000, 31: 247 – 25
Brown, A. (2020). Phytochemical analysis of Chromoleaa odorata leaves. Journal of Plant Research, 25(3), 123-135.
Cahyo, A. S. D., Oktavia, S., & Ifora. (2021). Anti-Inflammatory and Analgesic Potential of Chromolaena odorata: A Review. Sciences and Medicine, 6, 9, pp. 1–13
Fatema, N. (2013). Antioxidant and cytotoxic activities of Ageratum conyzoides stems,International Current Pharmaceutical Journal. 2,2, pp. 33-37.
Garcia, E. (2017). Saponins in traditional medicinal plants: A review. Journal of Ethnopharmacology, 40(2), 87-102.
Gislence GF, Nascimento JL, Paulo CF and Giuliana LS: Antibacterial
Gislence, G.F., Nascimento, J.L., Paulo, C.F. & Giuliana L.S (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant Bacteria. Brazilian J. of Microbiology. 31, pp. 247-256.
Harborne, J.B. (1973) Phytochemical Methods. Chapman and Hall Ltd., London, pp. 49-188.
Huang, Q., Lu, G., Shan, H.M., Chung, M.C.& Ong, C.N. (2007). Anticancer properties of anthraquinones from rhubarb. Med. Res. Rev. 27, 5, pp. 609-30.
Hunter, J.E., Zhang, J.& Kris-Etherton, P.M. (2009). Cardiovascular disease risk of dietary stearic acid compared with other saturated fatty acids. A systemic review. American Journal of Clinical Nutrition, 9,1, pp. 46-63.
Igboh, M.N., Ikewuchi, C.J., & Catherine, C. (2009). Chemical Profile of ChromolaenaodorataL. (King and Robinson) Leaves. Pakistan Journal of Nutrition, 8, pp. 521-524.
Ijioma, S.N. (2015). Blood coagulation tests and platelets counts in diabetic rats treated with Ficussur, Jatrophatanjorensis,
Mucunapruriens and Chromolaenaodorata Leaf Extracts. International Blood Research and Reviews, 3, 1, pp. 47-53.
Johnson, M. (2019). Tannins: Occurrence, biosynthesis, and potential health benefits. Annual Review of Food Science and Technology, 15(4), 210-225.
Kumar, M., Manish, K.G., Anit, S. & Goel, R.J (2013). Healing effects of Musa sapientum var. Paradisiacal in diabetic rats with co-occuring gastric ulcer, cytokines and growth factor by PCR amplification. BML Complementary and Alternative Medicone, 13, pp. 305.
Lai, P.K. & Roy, J. (2004). Antimicrobial and chemopreventive properties of herbs and spices. Curr Med Chem. 11, 11, pp. 1451-60.
Lee, S. (2021). Cardiac glycosides in plants: Pharmacological properties and therapeutic applications. Phytotherapy Research, 28, 1, pp. 45-56.
Lewis, D., Field, W., Shaw, &G. (1999). A natural flavonoid present in unripe plankain banana pulp protects the gastric mucosa from aspirin-Induced erosion. Journal of Ethnorpharmacology, 65, 3, pp. 283-6.
Lijana, R.C., & Williams, M.C. (1986). The effects of antibiotics on hemolytic behavior of red cells. Cell Biophys, 4, pp. 223-42.
Motamedi, h., Safary, M.S., Seyyednegad, S.M &ziziphus S.C. (2009). A native of antimicrobial agents. Asian J. Plant Sci. 8, pp. 187-190.
Ngozi, I.M., Jude, I.C. & Catherine, I.C., (2009). Chemical profile of Chromolaena odorata L. (King and Robinson) leaves. Pak. J. Nutr. 8, pp. 521–524
Ntie-Kang F., Amoa, P., Lifonga, L. L., Ndom, J. C., Sippi, W., Mbaze,& L.M. (2014). The potential of antimalarial compounds derived from African medicinal plants. Part 2: A pharmacological evaluation of non- alkaloid and non terpenoids. Malaria Journal, 13, pp.81
Nweze, J. A., Nweze, E. I., Nweze, B. A., & Ekejiuba, E. S. (2021). Antimalarial Activity of Chloromolaena odorata Leaf Extract in Mice Infected with Plasmodium berghei. BioMed Research International,, 6622485. https://doi.org/10.1155/2021/6622485
Ogunwande, I. A., Olawore, N. O., Ekundayo, O., & Walker, T. M. (2018). Antimicrobial activity of the essential oil of Chloromolaena odorata (L.) King & H.E. Robins from Nigeria. Natural Product Research, 32, 3, pp. 344–349. https://doi.org/10.1080/14786419.2017.1287634
Okigbo, R.N. & Mmeka, E.C. (2008). Antimicrobial effects of three tropical plant extracts on Staphylococcus aureus, Escherichia coli and Candida albicans. Afr J Tradit Complement Altern Med. 5, 3 pp. 226-9.
Orieke, D.,Ohaeri, O.C., Ijeh, I.I.& Ijioma, S.N. (2019). Semen quality, hormone profile and histological changesin male albino rats treated withCorchorusolitoriusleaf extract. Avicenna Journal of Phytomedcine, 9,6, pp. 551-562.
Parekh, H., Jadeja, & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk. J. Biol., 29, pp. 205-210.
Sirinthipaporn, A, & Jiraungkoorskul, W. (2017). Wound Healing Property Review of Siam Weed, Chromolaenaodorata. Pharmacogn Rev. 11, 21, pp. 35-38.
Smith, J. (2018). Alkaloids: Pharmacological significance and therapeutic applications. Natural Product Reports, 12(, 3, pp. 150-165.
Tapsell, L.C. (2006) Health Benefits of Herbs and Spices: The Past, the Present, the Future. Medical Journal of Australia, 1, pp. 4-24.
Taylor, R. (2016). Phenolic compounds in plant foods: Chemistry, bioavailability, and health effects. Journal of Food Composition and Analysis, 22, 2, pp. 123-135.
Trease, G.E. & Evans, E.C. (1985). A Textbook of Pharmacognosy, 13th Edition. English Language Book Society. BailliereTindall, Britain, 378, pp. 386-480.
Zhang, Y., Yao, X.B., Bao, B.L.,& Zhang, Y. (2006). Anti-fatique, antilipidaemia activity of a triterpenoid-rich extract from Chinese bamboo shavings. Phytother. Res, 20, pp. 33
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