Isolation and Characterization of Nonanoic Acid from Ethyl Acetate Extract of Adenodolichos paniculatus (hua) Hutch. & Dalz (Fabaceae)

Abstract

Adenodolichos paniculatus leaves were collected, identified, dried and pulverized.  The pulverized plant material was subjected to microwave-assisted extraction using n-hexane, chloroform, ethyl acetate and methanol. Column chromatography of the ethyl acetate extract led to a number of fractions. TLC finger printing and the spraying reagent (10  H₂SO₄) was used to study the different fractions. This isolation and purification afforded a yellow crystalline powder which was subjected to physical (melting point determination), and spectroscopic identification using IR, 1D, and 2D-NMR. The compound was identified as nonanoic acid.