Effect of Ethanolic Extract of Bryophyllum pinnatum Leaves On Haematological Parameters of Albino Wistar Rats Exposed to Cadmium Chloride
Keywords:
Toxicity, cadmium, hemoorhoids, hematemesis treatment, Bryophyllum pinnatumAbstract
Authors: Dorathy Edet Etim, John Cookey Ekpo, Aniebiet Mmekuwem Essien and Imaobong Nyambi Akpet
Received: 15 September 2022/Accepted 02 December 2023
This study was undertaken to investigate the effect of ethanolic extract of Bryophllum pinnatum leaves on haematological parameters of male albino Wistar rats exposed to cadmium chloride. seventeen (17) male albino Wistar rats were divided into five (5) groups consisting of five (5) rats each: Those in groups 1 and 2 were labelled as normal and negative control respectively. Experimental animals from groups 2 to 5 were treated with groups1ml of 26mg/kgbwt of cadmium chloride for seventeen (17) days. Those animals in test groups (3, 4 and 5) were also treated with 1ml of 100 mg/kgbwt, 200mg/kg/bwt and 300mg/kgbwt ethanolic extract of Bryophyllumpinnatum respectively for r twenty-one (21) days. After the exposure to CdCl2 solution for 17 days. The result obtained showed that there was no significant increase (P>0.05) in red blood cell (RBC), and PCV, non-significant while a slight decrease (P>0.05) in HGB and MCHC parameters were observed for the animals in group 1 (the normal control group) compared to those in the negative control group (group 2). There was a significant increase in PLT in the negative control group compared to the normal and test group members. Therefore the plant extract has the potency of treating menorrhagia, haemorrhoids and hematemesis and can be employed after satisfactory pre and post-clinical screenings.
Downloads
References
Andjelkovic, M., Djordjevic, A. E., Antonijevic, E., Antonijevic, B., Stanic, M., Stevuljevic, J. K., Kalimanovska, V. S., Jovanovic, M., Boricic, N., Wallace, D. & Bulat, Z. (2019). Toxic effect of acute cadmium and lead exposure in rat blood, liver and kidney. International Journal of Environmental Research and Public Health, 16(2), 274; https://doi.org/10.3390/ijerph16020274.
Donmez, H. H., Donmez, N., Kısadere, I. & Undag, I. (2019) Protective effect of quercetin on some hematological parameters in rats exposed to cadmium, Biotechnology and Histochemistry, 94:5, pp. 381-386, doiI: 10.1080/10520295.2019.1574027.
Mekkawy, I. A. A., Mahmoud, U. M., Wassif, E. T & Naguib, JM. (201). Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E. Fish Physiol Biochem 37, 71–84 (2011). https://doi.org/10.1007/s10695-010-9418-3.
Demir, S. & Oner, G. (1995). The effect of cadmium on the fragility of red blood cell. Journal of Islamic Academy of Sciences 8, 2, pp. 73-78.
Onwuka, F. C., Erhabor, O., Eteng, M. U. & Umoh, B. (2010). Ameliorative effect of cabbage extract on cadmium-induced changes on hematology and biochemical parameters of albino rats. Toxicology and Environmental Health Science, 2, 2, pp. -16, doi.org/10.5897/JTEHS.9000004
Asiwe, E. S., Igwe, C. U., Onwuliri, V. A., Iheanacho, K. M. E. & Iheancho, J. N. (2021). Characterization of the chemical composition of Bryophyllum pinnatum leaf ethyl acetate fraction. Asian Journal of Advanced Research and Reports, 51, pp. 15-24
doi: 10.9734/ajarr/2021/v15i430387
Ekwumemgno, P. A., Eddy, N. O. and Omonyi, I. K. (2013). Decontamination of heavy metals in polluted soil by phytoremediation using Bryophyllum pinnatum. Web Conferences, 1, 3004.
Garg, R., Garg, R., Eddy, N. O., Almohana, A. I., Fahad, S., Khan, M. A. and Hong, S. H. (2022). Biosynthesized silica-based zinc oxide nanocomposites for the sequestration of heavy metal ions from aqueous solutions. Journal of King Saud University-Science https://doi.org/10.1016/j.jksus.2022.101996.
Eddy, N. O. and Ekop, A. S. (2005). Study on the adsorption capacity of some animal shells for heavy metals. African Journal of Environmental Pollution and Health 4(1): 33-37.
Eddy, N. O. and Ukpong, I. J. (2005). Heavy metal concentration in upper Calabar River sediments, South Eastern Nigeria. African Journal of Environmental Science and Health4(1): 33-37.
Bruneton, J. (1995). Pharmacognosy, phytochemistry, Medicinal plants. pp.. 265 380. France: Lavoisier Publishing Co.
Bry, L., Chen, P. C. & Sacks, D. B. (2001). Effects of haemoglobin variants and chemically modified derivatives on assays for glycochemoglobin [Review]. Clinical Chemistry; 47, pp. 153-63.
Klaassen, C. D., Liu, J. & Diwan, B. A. (2009). Medicinal plant of India with Anti-diabetic potential. Toxicology and Applied Pharmacology. 238, 3, pp. :215-220.
Kotosonis, F. N. & Waasen, C. D. (1977). Toxicity and distribution of cadmium administered rats at sub-lethal dose. Toxicol. Appl. Pharmacol. 46, pp. 39-54.
Lang, I. A., Galloway, T. S, Scarlett, A., et al., (2008). “Association of urinary bisphenolA concentration with medical disorders and laboratory abnormalities in adults” JAMA 300, 11, pp. 1303-1310.
Ojewole, J. A. O. (2005). An antinociceptive, anti-inflammatory and antidiabetic effects of Bryophyllumpinnatum (Crassulaceae) leaf aqueous extract. Journal of Ethnopharmacology 99, pp. 13-19.
Prescott, L. M., Harley, J. P. & Klein, D. A. (2000). Microbiology, 4th Edition, McGraw-Hill Inc. pp. 978.
Principe, P. P. (1991). Valuing the biodiversity of medicinal plants. In: Akeele O., Heywood V., Synge H. (eds), the conservation of medicinal plants, pp. 79-124. Cambridge University press. Cambridge.
Rajagopal, K. & Sasikala, K. (2008). Antihyperglycaemic and antihyperlipidaemic effect of Nymphaeastellatainalloxan-induced diabetic rats, Singapore Medical Journal, 49, pp. 137-141.
Rao, B. R. R. (1999). Medicina plants for dry areas. In: Singh, R. P. and Osman, M. (eds), Sustainable alternate land use systems for drylands, pp. 139-156.
Wink, M. (1999). Introduction: biochemistry, role and biotechnology of secondary products. In Wink, M. edition, Biochemistry of Secondary product metabolish. CRC Press, boca Raton, FL., pp. 1-16.
Worobetz, R. J., Hilsden, E. A. Shaffer, J. B., Simon, P. & Para, V. G. (2005). The Liver: The basis of diseases and approach to management. G.I.T. Textbooks, UK., pages pp.462-475.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 The Journal and the author
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
