Chemical Information from GCMS Analysis of Acetone-Ethanol Ex-tract of Piper guineense Leaf. Part 2

Abstract

The need to know the different constituents of Piper  guineense  leaf extract through the use of different solvent is remarkable because solvents play significant role in the constituent that can be extracted from planr materials. GCMS analysis of Acetone-ethanol extract of  Piper guineense leaves indicated twenty one peaks which represented  2,3,6,7,7a-hexahydro-7a-methyl-5H-indene-5-one, alpha-cubebene, 1-ethenyl-1-methyl-2,4-bis(1-methylethenyl)-cyclohexane, caryophyllene, alpha caryophyllene, megastigma-7(E),9, 13-triene, eudesdma-4(14),11-diene, azulene, epsilon muurolene, di-t-butylacetylene, -cyclopentene -1-ethanol, 2,24-trimethyl, 2,6,6- trimethyl-3-(phenylthiol)cyclohep-4-enol, hexadecenoic acid, nonadecanoic acid, octadecanoic acid, cyclohexylidenecyclohexane,, pyrrolo[3,2-c]pyridine-4(5H)-one, 4,5-dihydrobenzo[1,2-c:3,4-c’]bis[1,2,5]oxadiazole, piperidine, 3,7-diacetamidophenoxathin and 4-(6-methoxy-3-mythyl-2-benzofuran)-3-buten-2-one respectively. The most abundant components of the extract was di-t-butylacetylene (17.36%) and ,5-dihydrobenzo[1,2-c:3,4-c’]bis[1,2,5]oxadiazole (12.95%). Most of the  identified compounds were reported to possess strong biological activities. Compounds that has not been reported from GCMS analysis of the plant leaves using other solvent were also observed which confirmed that the missed  solvent (acetone-ethanol) is more effective in extracting phytochemicals in Piper guineense leaves than either of the solvent.