Evaluation of Acute Toxicity and Antidiarrheal Activity of Hunteria umbellata Mesocarp Extract: Preclinical Study
Keywords:
Diarrhea, Hunteria umbellate, secretion, electrolyte, gastrointestinalAbstract
Communication in Physical Sciences, 2024, 11(2): 345-354
Authors: Onyinyechi Uloma Akoh*, Henry Nnanyere Nwigwe , Stella Mbanyeaku Ufearoh
Received: 23 January 2024/Accepted: 02 May2024
Diarrhea, characterized by increased water content in stools due to disruptions in intestinal physiological processes, poses significant health risks worldwide. Hunteria umbellata, a widely used medicinal plant in traditional medicine, holds the potential for treating various ailments, including diarrhea. This study aimed to evaluate the acute toxicity and antidiarrheal activity of Hunteria umbellata mesocarp extract. Acute toxicity assays (Phase 1 and Phase 2 LD50) conducted on mice revealed LD50 values greater than 5000 mg/kg body weight, indicating a wide safety margin. Subsequent antidiarrheal activity assessments using different models showed significant inhibition of diarrhea. In the charcoal meal transit model, the extract at 1000 mg/kg exhibited the highest inhibitory activity (49.89%). In the castor oil-induced model, the extract at 1000 mg/kg demonstrated the highest inhibition of diarrhoea (86.81%). Furthermore, in the castor oil-induced gastrointestinal fluid accumulation model, the extract at 500 mg/kg exhibited the highest antidiarrheal activity (56.28% inhibition). These findings support the potential therapeutic use of Hunteria umbellata mesocarp extract in managing diarrhea. Further preclinical and clinical studies are warranted to validate its efficacy and safety for human use. The results collectively suggest that the extract could serve as a safe and effective antidiarrheal agent, offering a promising alternative for diarrhea management.
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References
Akter S, Sarker A & Hossain S. (2013). Antidiarrhoeal activity of rind of Punica granatum, Int Curr Pharm J.; 2, 5, pp. 101–104.
Awouters F, Niemegeers C.J.E, Lenaerts F.M & Janssen P.A.J. (1978). Delay of castor oil diarrhoea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm. Pharmacol, 30, pp. 41-45.
Bern M.J, Sturbaum C.W, Karayalcin S.S, Berschneider H.M, Wachsman J.T & Powell D. (1989). Immune system control of rat and rabbit colonic electrolyte transport. Role of prostaglandins and enteric nervous system. J Clin Invest;83, 6, pp. pp. 1810-1829.
Chen J, Wan C.M,Gong S.T, Fang F , Sun M, Qian Y , Huang Y, Wang B.X, Xu C.D, Ye L.Y, Dong M, Jin Y , Huang Z.H, Wu Q.B, Zhu C.M, Fang Y.H, Zhu Q.R &Dong Y.S. (2018). Chinese clinical practice guidelines for acute infectious diarrhea in children. World J Pediatr. 14, 5, pp. 429-436.
Davidson-Hunt I. (2000). Ecological ethno botany: stumbling toward new practices and paradigms. Model Assisted Statistics and Applications Journal, 16, pp. 1–13
Di Carlo G, Autore G, Izzo A.A, Maiolino P, N, Mascolo P & Viola (1993). Inhibition of intestinal motility and secretion by flavonoids in mice and rats: Structure activity relationships. J Pharm Pharmaco, 45, 12, pp. 1054–1059
Emudainohwo J, Erhirhie E & Moke E. (2015). Anti-diarrheal activity of the aqueous leaf extract of Ageratum conyzoides in wistar rats. J Appl Sci Environ Manag, 19, 2, pp. 169–75.
Ezeja M.I, Ezeigbo I.I, Madubuike K.G, Udeh N.E, Ukweni I.A, Akomas S.C, & Ifenkwe D.C. (2012). Antidiarrheal activityof pterocarpus erinaceus methanol leaf extract in experimentally-induced diarrhea. Asian Pacific J Trop Med 5, 2, pp. 147-50.
Falodun A, Nworgu Z & Ikponmwonsa M. O. (2006). Phytochemical components of H. umbellate (K. Schum) and its effect on isolated non-pregnant rat uterus in oestrus. Pak J Pharm Sci, 19, 3, pp. 256–258.
Galvez J, Crespo M.E, Jimenez J, Suarez A & Zarzuelo A. (1993). Anti-diarrhoeic activity of quercitrin in mice and rats. J Pharm Pharmacol. 45, 2, pp. 157–159
Gills L.S. (1992). Ethnomedicinal uses of plants in Nigeria. Benin City: Uniben Press; p. 134.
Horton E.W, Main I.H, Thompson C.J & Wright P.M. (1968). Effect of orally administered prostaglandin E1 on gastric secretion and gastrointestinal motility in man. Gut., 9, 6, pp. 655–658.
Komal, Kumar S & Rana A.C. (2013). Herbal approaches for diarrhea: a review. Int Res J Pharm 4, 1, pp. 31–38.
Lorke D. (1983). A new approach to tropical acute toxicity testing. Arch. Toxicol., 54, pp. 275-287.
Meite S , N’guessan J, Bahi C, Yapi H, Djaman A & Guina F.G. (2009). Antidiarrheal activity of the ethyl acetate extract of Morinda morindoides in rats. Tropical Journal of Pharmaceutical Research. 8, 3, pp. 201–7.
Mascolo N, Izzo A.A, Autore G, Barbato F & Capasso F. (1994). Nitric oxide and castor oil-induced diarrhea. J. Pharmacol. Exp. Ther., 263, pp. 291-295.
Null C , Stewart C.P, Pickering A.J, Dentz H.N, Arnold B.F, Arnold C.D, Benjamin-Chung J, Clasen T, DeweyK.G, Fernald L.C.H, Hubbard A.E, Kariger P, Lin A, Luby S.P, Mertens A, Njenga S.M, Nyambane G, Ram P.K & Colford J.M. (2018). Effects of water quality, sanitation, handwashing, and nutritional interventions on diarrhoea and child growth in rural Kenya: a cluster-randomised controlled trial. Lancet Glob Health. 6, 3, pp. 316-329.
Oliver-Bever B. (1986). Medicinal plants in tropical West Africa. Cambridge: Cambridge University Press; p. 60–2.
Rahman A, Hasan S.N, Sampad K.S & Das A.K. (2011). Antinociceptive, anti diarrheoal and cytotoxic activities of Rhizophora mucronata Lamk. Pharmacologyonline, 1, pp. 921-929.
Rahman M.M, Islam A.M.T, Chowdhury M.A.U, Uddin M.E & Jamil A. (2012). Antidiarrheal activity of leaves extract of Microcos paniculata Linn in mice. Int. J. Pharm., 2, pp. 21-25.
Robert A, Nezamis J.E, Lancaster C, Hanchar A.J & Klepper M.S (1976). Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostaglandins, 11, 5, pp. 809–828
Sofowora A. (1993). Medicinal plants and traditional medicine in Africa. 2nd ed. Netherlands: Spectrum Books Limited, Ibadan; p. 150.
Uddin S.J. (2005). Antidiarrhoeal activity of the methanol extract of the barks of Xylocarpus moluccensis in castor oil- and magnesium sulphate-induced diarrhoea models in mice. J Ethnopharmacol 101, pp. 139–143. Wenzl H.H. (2012). Diarrhea in chronic inflammatory bowel diseases. Gastroenterol Clin North Am. 41, 3, pp. 651-75.
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