Gas Chromatography-Mass Spectrometry Vernonia Hymenolepis Analysis of the Solvent-Solvent Extract of Vernonia Hymenolepis Leaves

Abstract

Edet O. Odokwo and Ngozi M. Uzoekwe

The solvent-solvent extraction and gas chromatography-mass spectrometry analysis of the leaves of Vernonia hymenolepis has been carried out. The dried leaves were extracted using n-hexane and partitioned with absolute ethanol. The concentrated ethanol fraction, EVH was analyzed using a Gas Chromatography-Mass Spectrometry, GC-MS instrumentation. The present study has identified thirty-one (31) phytoconstitutions in the volatile extract of Vernonia hymenolepis. 2-Methyl-Z,Z-3,13-octadecadienol (3.16%); cis-verbenol (4.13%); 6-nonynoic acid (2.39%); 1H-pyrrolo[3,4-d]pyrimidine-2,5-di one, 4,6-bis(4-hydroxyphenyl)-1-methyl-3,4,6,7-tetrahydro- (4.91%); myo-Inositol, 4-C-methyl- (9.84%); ethanol, 2-phenoxy-, acetate (3.83%); cyclododecane (2.61%); 2-piperidinone, N-[4-bromo-n-butyl]- (3.52%); tetradecanoic acid (2.05%); hexadecanoic acid methyl ester (5.97%); n-hexadecanoic acid (9.85%); methyl stearate (2.83%); 1-nonadecene (4.97%); octadecanal (2.06%); tetracosane(3.81%); eicosane (2.85%); 2H-indol-2-one, 1,3-dihydro-5-hydroxy- (4.99%) and 1-octen-3-yne (2.07%) were identified as the major constituents.