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Communication in Physical Sciences 2020, 5(3): 325-337
Authors: O.V. Ikpeazu, *Ifeanyi. E. Otuokere and K. K. Igwe
Received 20 May 2020/Accepted 23 June 2020
Knowledge of bioactive components of a given plant materia; is essential in furnishing information on possible applications. Therefore, this study was carried out to identify bioactive components in ethanol extract of Combretum hispidum roots using GCMS analysis. Results obtained from GCMS analysis of ethanol extract of Combretum hispidum roots indicated the presence of benzoic acid N'-(4,4,5,5,6,6,6-heptafluoro-3-oxo-1-phenylhex-1-enyl)-hydrazide, 1-(4-methyl-6-methoxy-2-quinolyl)-3,3'-dimethyl-(4,5'-bipyrazol)-5-ol, trans-1,2-Diphenyl-1-chloro-2-methylthio-ethene, 3-acetyl-3-demethylthiocolchicine, 1-anthracenecarboxaldehyde, 5,8,8a,9,10,10a-hexahydro-2,3-dihydroxy-10a-methyl-4-(1-methylethyl)-6-(4-methyl-, 1H-benzimidazole, 2-(2,2- dimethylpropyl)-, 1,3-benzoxathiol-2-one, 5- hydroxy-4,7-dimethyl-6-nitro-, bis(ditrifluoromethyldiphosphino)sulfide, benzeneethenylamine, 3,4-dihydroxy-N-isopropyl-, voachalotine oxindole, acetate (ester), ethanone, 1-(3-chloro-5,6-dihydro-1,4-oxathiin-2-yl)-, benzenetridecanoic acid, 3-chloro-4-methoxy-, 3-methoxy-2-(9-methyldecyl)-5-(4- methylpentyl)phenyl ester, ethyl 4,4-dimethyl-5- oxo-tetrahydrofuran-3-carboxylate, N-(4- bromophenylsulfonyl)aziridine-2,2-dicarboxylic acid diethyl ester, 1,3,5-triazin-2-amine, N,Ndihexyl-4,6-bis(2-naphthalenylthio)-, 1[5'- (hydroxymethyl)furfuryl] pyrrolidine, terephthalic acid, butyl cycloheptyl ester, 1H-pyrazole, 4,5- dihydro-1,3-diphenyl-, succinic acid, 2,2,3,3,4,4,5,5-octafluoropentyl 1-cyclopentylethyl ester. Each of the identified compounds in the extract have been documented to exhibit active pharmaceutical/medicinal activities. It was concluded that the bioactive compounds support the use of C. hispidum roots in the treatment of diseases like cancer, anaphylactic shock, renal failure, diabetes and hypertension.
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Agra, M.F., Freitas, P.F. & Barbosa-Filho, J.M. (2007). Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia,17, pp. 114– 140.
Ademola, I.O. & Eloff, J.N. (2010). In vitro anthelmintic activity of Combretum mole (R. Br. ex G. Don) (Combretaceae) against Haemonchuscontortusova and larvae. Vetenary. Parasitology, 169, pp. 198–203.
Aderogba, M.A., Kgatle, D.T., McGaw, L.J.& Eloff, J. N. (2012). Isolation of antioxidant constituentsfrom Combretum apiculatumsubsp. apiculatum. South African Journal of Botany,79, pp. 125–131.
Asres, K., Bucar, F., Knauder, E., Yardley, V.,Kendrick, H., Croft, S.L. (2001). In vitro antiprotozoal activity of extract and compounds from the stem bark of Combretum molle. Phototherapy. Research, 15, pp. 613–617.
Atindehou, K.K., Schmid, C., Brun, R., Kone, M.W. & Traore, D. (2004). Antitrypanosomal and antiplasmodial activity of medicinal plants from Côte d’Ivoire. Journal of Ethnopharmacology. 90,221–227.
Chaabi, M., Benayache, S., Benayache, F., N’Gom, S., Koné, M., Anton, R., Weniger, B. &Lobstein, A. (2008). Triterpenes and polyphenols from Anogeissusleio carpus (Combretaceae). Biochemistry and. Systematic Ecology,36, pp. 59–62.
Chika, A. & Bello, S.O. (2010). Antihyperglycaemic activity of aqueous leaf extract of Combretum micranthum (Combretaceae) innormal and alloxan-induced diabetic rats. Journal of Ethnopharmacology.129, pp. 34–37.
Connolly, B.S. & Lang, A.E. (2014). Pharmacological treatment of Parkinson disease: A Review. JAMA. 311, 16, pp. 1670-83.
Duke’s phytochemical and Ethnobotanical databases (2019). Available at https://data.nal.usda.gov
Edeoga, H.O., Okwu, D.E. & Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4, pp. 685–688.
Fyhrquist, P., Mwasumbi, L., Vuorela, P., Vuorela, H., Hiltunen, R., Murphy, C., Adlercreutz, H. (2006). Preliminary antiproliferative effects of some species of Terminalia, Combretum and Pteleopsis collected in Tanzania on some human cancer cell lines. Fitoterapia2 77, pp. 358–366.
Gansané, A., Sanon, S., Ouattara, L.P., Traoré, A., Hutter, S., Olivier, E., Azas, N., Traore, A. S. Guissou, I. P. & Sirima, S. B. (2010). Antiplasmodial activity and toxicity of crude extracts from alternatives parts of plants widely used for the treatment of malaria in Burkina Faso: Contribution for their preservation. Parasitology. Research,106, pp. 335–340.
Hoareau, L. & Da Silva, E. J. (1999). Medicinal plants: A re-emerging health aid. Eletronic. Journal of Biotechnology, 2, pp. 56–70.
Hyde, C.A.C &Missailidis, S.(2009). Inhibition of arachidonic acid metabolism and its implication on cell proliferation and tumour-angiogenesis, International Immunopharmacology, 9, 6, pp.701-715
Igwe, K.K., Nwankwo, P.O., Otuokere, I.E., Chika, I. &Amaku, F.J (2016): Studies on the medicinal plant Acalypha wilkesiana ethanol extracts phytocomponents by GC-MS analysis, Global Journal of Science Frontier Research, 16,2, pp. 48–55.
Ikpeazu, O.V., Otuokere, I.E. &Igwe, K.K. (In Press). GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum hispidum (Laws) (Combretaceae) leaves, Journal of Chemical Society of Nigeria.
Lall, N. & Meyer, J. J. M.(1999). In vitro inhibition of drug-resistant and drug-sensitive strains of Mycobacterium tuberculosis by ethnobotanically selected South African plants. Journal of Ethnopharmacology 66, pp. 347–354.
Martin, R .T., Roch, P. B., Van Luu-The, D.P. (2005) Inhibitors of Type 1 17beta hydroxysteroid Dehydrogenase With Reduced Estrogenic Activity: Modifications of the Positions 3 and 6 of Estradiol, Journal of Enzyme Inhibitionand Medicinal Chemistry, 20, 2, pp.153 -163.
Muthu, C., Ayyanar, M., Raja, N. & Ignacimuthu, S. (2006). Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. Journal od Ethnobiology and Enthnomedicine, 2, doi:10.1186/1746-4269-2-43.
National library of Medicine (2020) National Center for Biotechnology Information. PubChem Database, Methylguanidine. Available at https://pubchem.ncbi.nlm.nih.gov/compound/methylguanidine
Njume, C., Jide, A.A. & Ndip, R. N. (2011). Aqueous and organic solvent-extracts of selected South African medicinal plants possess an-
timicrobial activity against drug-resistant strains of Helicobacter pylori: Inhibitory and bactericidal potential. International Journal of Molecular Science, 12, pp. 5652–5665.
Otuokere, I. E., Okorie, D.O., Igwe, K.K. &Mathew, U.J. (2016) GCMS Determination of Bioactive Phytocompounds in Chromolaenaodorataleaf exract. Int J of Advances in Engineering Technology and Sciences, 2, 3, pp. 7-
Pietrovski, E.F., Rosa, K.A., Facundo, V.A., Rios, K., Marques, M.C.A. & Santos, A. R. S. (2006). Antinociceptive properties of the ethanolic extract and of the triterpene 3β,6β,16βtrihidroxilup-20(29)-ene obtained from flowers of Combretum leprosumin mice. Pharmacol. Biochem. Behav.83, pp. 90–99.
Westwood, I.M. (2010) Identification of Arylamine N-acetyltransferase Inhibitors as an Approach Towards Novel Anti-Tuberculars, Protein Cell., 1, 1, pp. 82-95.
Yan-qun, L., De-xin, K. & Hong, W. (2013). Analysis and evaluation of essential oil components of Cinnamon barks using GC-MS and FTIR spectroscopy. Elsevier: Industrial Crops and Products, 41, pp. 269 – 278