Gas Chromatography–Mass Spectrometric Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum hispidum (Laws) (Combretaceae) Root


  • O.V. Ikpeazu Abia State University, Uturu
  • *Ifeanyi E.Otuokere Michael Okpara University of Agriculture, Umudike, Nigeria
  • K.K.Igwe Michael Okpara University of Agriculture, Umudike, Nigeria


Bioactive compounds, Combretum hispidum, gas chromatography, mass spectrometry, roots


Communication in Physical Sciences 2020, 5(3): 325-337

Authors: O.V. Ikpeazu, *Ifeanyi. E. Otuokere and K. K. Igwe
Received 20 May 2020/Accepted 23 June 2020

Knowledge of bioactive components of a given plant materia; is essential in furnishing information on possible applications. Therefore, this study was carried out to identify bioactive components in ethanol extract of Combretum hispidum roots using GCMS analysis. Results obtained from GCMS analysis of ethanol extract of Combretum hispidum roots indicated the presence of benzoic acid N'-(4,4,5,5,6,6,6-heptafluoro-3-oxo-1-phenylhex-1-enyl)-hydrazide, 1-(4-methyl-6-methoxy-2-quinolyl)-3,3'-dimethyl-(4,5'-bipyrazol)-5-ol, trans-1,2-Diphenyl-1-chloro-2-methylthio-ethene, 3-acetyl-3-demethylthiocolchicine, 1-anthracenecarboxaldehyde, 5,8,8a,9,10,10a-hexahydro-2,3-dihydroxy-10a-methyl-4-(1-methylethyl)-6-(4-methyl-, 1H-benzimidazole, 2-(2,2- dimethylpropyl)-, 1,3-benzoxathiol-2-one, 5- hydroxy-4,7-dimethyl-6-nitro-, bis(ditrifluoromethyldiphosphino)sulfide, benzeneethenylamine, 3,4-dihydroxy-N-isopropyl-, voachalotine oxindole, acetate (ester), ethanone, 1-(3-chloro-5,6-dihydro-1,4-oxathiin-2-yl)-, benzenetridecanoic acid, 3-chloro-4-methoxy-, 3-methoxy-2-(9-methyldecyl)-5-(4- methylpentyl)phenyl ester, ethyl 4,4-dimethyl-5- oxo-tetrahydrofuran-3-carboxylate, N-(4- bromophenylsulfonyl)aziridine-2,2-dicarboxylic acid diethyl ester, 1,3,5-triazin-2-amine, N,Ndihexyl-4,6-bis(2-naphthalenylthio)-, 1[5'- (hydroxymethyl)furfuryl] pyrrolidine, terephthalic acid, butyl cycloheptyl ester, 1H-pyrazole, 4,5- dihydro-1,3-diphenyl-, succinic acid, 2,2,3,3,4,4,5,5-octafluoropentyl 1-cyclopentylethyl ester. Each of the identified compounds in the extract have been documented to exhibit active pharmaceutical/medicinal activities. It was concluded that the bioactive compounds support the use of C. hispidum roots in the treatment of diseases like cancer, anaphylactic shock, renal failure, diabetes and hypertension.


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Author Biographies

O.V. Ikpeazu , Abia State University, Uturu

Department of Biochemistry

*Ifeanyi E.Otuokere, Michael Okpara University of Agriculture, Umudike, Nigeria

Department of Chemistry

K.K.Igwe, Michael Okpara University of Agriculture, Umudike, Nigeria

Department of Vet. Biochemistry and Animal Production


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